Dictionary

misprime en · VERB

Etymology

From mis- + prime.

Meanings

1. To prime incorrectly.
   • It is also useful when using degenerate primers which may misprime and cause nonspecific products. 2007, Ralph Rapley, John M. Walker, Molecular Biomethods Handbook, page 310:
   • As described previously, primers are apt to dimerize or misprime, particularly in the absence of specific target. 2010, Stephen A. Bustin, The PCR Revolution: Basic Technologies and Applications, page 139:
   • Aberrant annealing has also been observed — either due to the use of the incorrect tRNA to misprime reverse transcription (Colicelli and Goff 1986a, b; Wakefield et al. 1995) or due to the priming of cellular mRNAs with short “tRNA-like” primers (Taylor and Cywinski 1984). 2013, Stuart LeGrice, Matthias Gotte, Human Immunodeficiency Virus Reverse Transcriptase, page 208:

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